Exogenous hydrogen sulphide alleviates nodule senescence in Glycine max-Sinorhizobium fredii symbiotic system

Hydrogen sulphide (H2S), as a signalling molecule, regulates the process of plant growth and development. H2S is involved in establishment legume rhizobial symbiotic relationship, but little known about role during nodule senescence. We studied function senescence Glycine max-Sinorhizobium fredii system. Physiological biochemical methods were used to modulate nitrogen fixation, structure, senescence-related protein gene expression. Our results indicated that decreased senescent number, increased nitrogenase (Nase) activity ureide content, further promoted accumulation NO3−-N, NH4+-N, total N, which caused biomass late steps symbiosis. alleviated by maintaining normal fixation structure nodules. Meanwhile, expression levels NifH NodGS proteins N metabolic enzyme activities enhanced Moreover, stimulated metabolism-related genes Finally, regulated These findings suggested prolonged lifespan N2 delaying senescence, will be important for increasing soybean yield agricultural sustainability